To solve the problem of Fine Particulate Matter (PM2.5) concentration prediction, a PM2.5 concentration prediction model was proposed. First, through introducing the comprehensive meteorological index, the factors of wind, humidity, temperature were comprehensively considered; then the feature vector was conducted by combining the actual concentration of SO2, NO2, CO and PM10; finally the Least Squares Support Vector Machine (LS-SVM) prediction model was built based on feature vector and PM2.5 concentration data. The experimental results using the data from the city A and city B environmental monitoring centers in 2013 show that, the forecast accuracy is improved after the introduction of a comprehensive weather index, error is reduced by nearly 30%. The proposed model can more accurately predict the PM2.5 concentration and it has a high generalization ability. Furthermore, the author analyzed the relationship between PM2.5 concentration and the rate of hospitalization, hospital outpatient service amount, and found a high correlation between them.